Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SUZ12

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
cell line
K562
cell type
Human immortalized myelogenous leukemia cell line
genotype
Control
chip antibody
Rabbit monoclonal anti-SUZ12 (Cat#3737, Cell Signaling Technology)
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6219549
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde. ChIP was preformed with the iDeal ChIP-seq kit for Histone marks (Diagenode) or the the iDeal ChIP-seq kit for Transcription Factors (Diagenode) Library preparation was performed with KAPA HyperPrep Kit for NGS DNA Library Prep (Roche) according to the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
42488664
Reads aligned (%)
93.5
Duplicates removed (%)
44.1
Number of peaks
2942 (qval < 1E-05)

hg19

Number of total reads
42488664
Reads aligned (%)
92.9
Duplicates removed (%)
45.3
Number of peaks
2697 (qval < 1E-05)

Base call quality data from DBCLS SRA