Antigen

Antigen Class

TFs and others

Antigen

SUZ12

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

cell line

K562

cell type

Human immortalized myelogenous leukemia cell line

genotype

Control

chip antibody

Rabbit monoclonal anti-SUZ12 (Cat#3737, Cell Signaling Technology)

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM6219549

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde. ChIP was preformed with the iDeal ChIP-seq kit for Histone marks (Diagenode) or the the iDeal ChIP-seq kit for Transcription Factors (Diagenode) Library preparation was performed with KAPA HyperPrep Kit for NGS DNA Library Prep (Roche) according to the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

42488664

Reads aligned (%)

93.5

Duplicates removed (%)

44.1

Number of peaks

2942 (qval < 1E-05)

hg19

Number of total reads

42488664

Reads aligned (%)

92.9

Duplicates removed (%)

45.3

Number of peaks

2697 (qval < 1E-05)